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Biphasic effect of Solanum nigrum fruit aqueous extract on vascular mesenteric beds in non-diabetic and streptozotocin-induced diabetic rats

1, 2, 1,2★, 3★ Corresponding author

  1. 1Molecular Medicine Research Center, 2Department of Physiology, School of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas
  2. 2Department of Physiology, School of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas
  3. 3Department of Pharmacognosy, Faculty of Pharmacy, Shahid Bahashti University of Medical Sciences, Tehran, Iran

CORRESPONDENCE

Nepton Soltani

Molecular Medicine Research Center

neptun.soltani@gmail.com

Volume 6, Issue 2 · pp. 148-152 · 2014 · DOI: 10.4103/0974-8490.129036

ABSTRACT

In Asia, Solanum nigrum fruit is traditionally used to manage, control, and treat diabetes. This study was carried out to investigate the endothelium and nitric oxide roles in Solanum nigrum-induced vasorelaxation in non-diabetic and diabetic rat vessels. Diabetes was induced by a single i.p. injection of streptozotocin. Eight weeks later, superior mesenteric arteries of non-diabetic and diabetic groups were isolated and perfused according to the McGregor method. Solanum nigrum fruit extract (SNE) at concentrations of 0.00001 to 0.6 mg/ml was added to the medium and perfusion pressure was recorded. Baseline perfusion pressure of diabetic group was significantly higher than non-diabetic rats in both intact and denuded endothelium. The low concentrations of SNE have vasodilatory effect in the diabetic and non-diabetic, but high concentrations of SNE produce initial significant contractions, followed by secondary relaxations in normal and diabetic rats. We observed vasorelaxation at low and high concentrations of SNE in both diabetic and non-diabetic groups after endothelium denudation. SNE-induced vasorelaxation in diabetic group is mediated by both endothelium and smooth muscle, but the relaxatory effect of SNE in non-diabetic group is not mediated by endothelium, and SNE has direct action on the smooth muscle. Although the part of SNE-induced relaxation in diabetic vessel was mediated by endothelium, nitric oxide didn’t play any role in this action, and maybe we can use SNE in the management of diabetes vessel complications in future.

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    Sohrabipour, S., Kharazmi, F., Soltani, N., & Kamalinejad, M. (2014). Biphasic effect of Solanum nigrum fruit aqueous extract on vascular mesenteric beds in non-diabetic and streptozotocin-induced diabetic rats. Pharmacognosy Research, 6(2), 148-152. https://doi.org/10.4103/0974-8490.129036

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    © 2014 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0