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The effect of 30% ethanolic extract of Indian propolis on replica of human dentin compared against commercially available desensitizing agent: A methodological SEM study in vitro

1, 1★, 1, 1★ Corresponding author

  1. 1Department of Public Health Dentistry, People’s Dental Academy, People’s University, Bhopal, Madhya Pradesh, India

CORRESPONDENCE

Nilesh Arjun Torwane

Department of Public Health Dentistry, People’s Dental Academy, People’s University, Bhopal, Madhya Pradesh, India

nealdip@gmail.com

Accepted: 21-11-2013.

Volume 6, Issue 2 · pp. 113-119 · 2014 · DOI: 10.4103/0974-8490.129026

ABSTRACT

This study evaluated the ability of 30% ethanolic extract of Indian propolis on dentinal tubule occlusion comparatively against CPP-ACP containing desensitizing agent GC tooth mousse. The specimens were prepared from 30 freshly extracted sound human third molars stored in 10% formalin (pH 7.0) at a room temperature. From each specimen, a sectioned sample (5 mm length × 5 mm width × 3.5 mm depth) was obtained including the cervical area. Samples were smoothened and wet-polished with 1000- and 1200-grit aluminum oxide abrasive paper and diamond pastes, in order to stimulate the clinical aspect of hypersensitive dentin cervical surfaces. All the specimens were randomly assigned to three groups (n = 10), according to dentin surface treatments. Negative control: Untreated specimens (n = 4) and pretreated with 6% citric acid (n = 6); Test Group: 30% ethanolic extract of Indian propolis (n = 10); Positive Group: GC Tooth Mousse (n =10). All the specimens were prepared for SEM analysis. GC tooth mousse promoted tubule occlusion by crystal-like deposits in the lumen of the tubules. While propolis created a thin, smooth layer over dentin surface. According to the SEM analysis, both desensitizing agent were able to occlude the dentinal tubules.

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    Hongal, S., Torwane, N. A., Goel, P., & Chandrashekar, B. (2014). The effect of 30% ethanolic extract of Indian propolis on replica of human dentin compared against commercially available desensitizing agent: A methodological SEM study in vitro. Pharmacognosy Research, 6(2), 113-119. https://doi.org/10.4103/0974-8490.129026

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    © 2014 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0