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Antiproliferative effects of Plumbago rosea and its purified constituent plumbagin on SK-MEL 28 melanoma cell lines

1, 2, 3, 3, 3★★ Corresponding author

  1. 1Department of Biotechnology, Kamaraj College of Engineering and Technology, Virudhunagar, Tamil Nadu
  2. 2Biogenix Research Centre, Trivandrum, Kerala
  3. 3Department of Biotechnology, Bharathidasan Institute of Technology Campus, Anna University, Tiruchirappalli, Tamil Nadu, India

CORRESPONDENCE

Sureshkumar Periyasamy

Department of Biotechnology, Bharathidasan Institute of Technology Campus, Anna University, Tiruchirappalli, Tamil Nadu, India

sureshbiotech2003@yahoo.co.in

Volume 6, Issue 4 · pp. 312-319 · 2014 · DOI: 10.4103/0974-8490.138280

ABSTRACT

Plumbago rosea is used in traditional systems of medicine for the preparation of formulations used for treating inflammations, cough, bronchitis, and gastrointestinal disorders, and also in conjunction with cancer chemotherapy. In the present study, the cytotoxic and anti-proliferative effects of plumbagin, and the ethanolic root extract of P. rosea (ETPR) was evaluated on SK-MEL 28 melanoma cell lines and human lymphocytes. MTT and apoptotic assays were used for the evaluation of cytotoxic and anti-proliferative effects, respectively. In addition, the effect of Plumbagin and ETPR in down regulation of BCL-2 expression is investigated using RT-PCR analysis. Both plumbagin and ETPR dose-dependently decreased the cell viability more potently in melanoma cell lines. P. rosea extract demonstrated significant synergy in inhibiting BCL-2 expression than plumbagin. Moreover plumbagin showed more toxicity in human lymphocytes. Plumbagin has anti-cancer potential, but the side effects limits its use; yet plumbagin, in combination with other ingredients in Plumbago rosea extract, displays significant synergy leading to a stronger anticancer effect with significantly less toxicity.

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    Anuf, A. R., Ramachandran, R., Krishnasamy, R., Gandhi, P. S., & Periyasamy, S. (2014). Antiproliferative effects of Plumbago rosea and its purified constituent plumbagin on SK-MEL 28 melanoma cell lines. Pharmacognosy Research, 6(4), 312-319. https://doi.org/10.4103/0974-8490.138280

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    © 2014 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0