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Antiallergic effect of an aqueous leaf extract of Pistia stratiotes in murine model of ovalbumin-induced allergic conjunctivitis

1, 2★, 3, 4, 4, 3★ Corresponding author

  1. 1Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, 2Department of Optometry, School of Physical Sciences, University of Cape Coast, Cape Coast
  2. 2Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi
  3. 3Department of Optometry, School of Physical Sciences, University of Cape Coast, Cape Coast
  4. 4Department of Pathology, Komfo Anokye Teaching Hospital, Kumasi, Ghana

CORRESPONDENCE

George Asumeng Koffuor

Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi

gkoffuor@yahoo.com

Volume 6, Issue 4 · pp. 274-279 · 2014 · DOI: 10.4103/0974-8490.138243

ABSTRACT

The aim was to investigate the antiallergic effect of an aqueous leaf extract of Pistia stratiotes (ALPS) in a murine model of ovalbumin (OVA)-induced allergic conjunctivitis (AC). Prior to topical challenge (instillation of 1.5 mg OVA in 10 μL phosphate buffered saline into their conjunctival sacs) to induce AC, groups of sensitized Imprinting Control Region mice (injected IP, on day 1 and 7, with 0.2 ml solution of 100 μg OVA and 0.01 mg aluminum hydroxide in phosphate buffered saline), were treated with 5 mg/kg cetirizine, 10, 50 or 100 mg/kg of ALPS, or 2 ml/kg normal saline per os. Conjunctival redness, lid edema, tearing and lid scratching (clinical symptoms of AC) were scored. Serum OVA specific immunoglobulins were determined using ELISA. Histopathological assessment of the conjunctival mucosal tissue was conducted. The extract was screened for secondary plant metabolites. Pretreatment with the extract significantly (P ≤ 0.05–0.01) and dose-dependently reduced the scores for clinical symptoms, which were marked in vehicle-pretreated mice. Pretreatment also lowered (P ≤ 0.01–0.001) serum OVA specific immunoglobulins. Mast cell infiltration and degranulation in conjunctival stroma (measured by an inflammatory score) in histopathological studies was also significantly low (P ≤ 0.05–0.01) on pretreatment. The ALPS exhibited interesting antiallergic activity and hence could be useful in managing AC.

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    Abokyi, S., Koffuor, G. A., Kyei, S., Asiamah, E. A., Atobiga, C. N., & Awuah, A. (2014). Antiallergic effect of an aqueous leaf extract of Pistia stratiotes in murine model of ovalbumin-induced allergic conjunctivitis. Pharmacognosy Research, 6(4), 274-279. https://doi.org/10.4103/0974-8490.138243

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    © 2014 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0