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Autonomic Receptors and Nitric‑Oxide Involvements in Mediating Vasorelaxation Effect Induced by Syzygium polyanthum Leaves Extract

1★, 1★ Corresponding author

  1. 1Department of Fundamental Dental and Medical Sciences, Kulliyyah of Dentistry, International Islamic University Malaysia, Indera Mahkota, Pahang.
  2. 1Biomedicine Program, School of Health Sciences, Universiti Sains Malaysia, Kelantan, Malaysia.

CORRESPONDENCE

Azlini Ismail

Department of Fundamental Dental and Medical Sciences, Kulliyyah of Dentistry, International Islamic University Malaysia, Indera Mahkota, Pahang.

Volume 9, Issue 5s · pp. s9-s14 · 2017 · DOI: 10.4103/pr.pr_69_17

ABSTRACT

Context: Syzygium polyanthum (Wight) Walp leaves are traditionally used by Malays for treating hypertension. Our previous study showed that aqueous extract ofS. polyanthum(AESP) and methanolic extract ofS. polyanthum (MESP) extracts of S. polyanthumleaves significantly reduced blood pressure of normotensive Wistar‑Kyoto (WKY) and spontaneously hypertensive rats (SHR).Aims: This study aimed to investigate their vasorelaxation potential and the possible involvement of autonomic receptors and nitric oxide in mediating their effect. Settings and Design: Both extracts will be tested on isolated thoracic aorta rings of WKY and SHR. The involvement of autonomic receptors and nitric oxide will be elucidated using respective blockers.Materials and Methods: Isolated thoracic aorta rings from WKY and SHR were mounted onto myograph chambers to measure changes in the aorta tension. Increasing concentrations of AESP and MESP, from 1 µg/ ml to 10 mg/ml were added onto the myograph chambers. Blockers such as atropine (1 µM), phentolamine (1 µM), propranolol (1 µM), and Nω‑nitro‑l‑arginine methyl ester (100 µM) were preincubated before addition of extracts to check for involvement of muscarinic, α‑ and β‑adrenergic receptors (AR) as well as nitric oxide, respectively. Statistical Analysis Used: Two‑way ANOVA, followed by post hoc Bonferroni test was used, where P < 0.05 (two‑tailed) was considered statistically significant.Results: AESP and MESP caused significant vasorelaxations through nitric oxide pathway. The former was mediated through α‑AR while the latter was mediated by β‑adrenergic and muscarinic receptors.Conclusion: Vasorelaxation effect by AESP and MESP involved nitric oxide pathway which is possibly mediated by the autonomic receptors.

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    Ismail, A., & Ahmad, W. A. N. W. (2017). Autonomic Receptors and Nitric‑Oxide Involvements in Mediating Vasorelaxation Effect Induced by Syzygium polyanthum Leaves Extract. Pharmacognosy Research, 9(5s), s9-s14. https://doi.org/10.4103/pr.pr_69_17

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    © 2017 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0