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Chemical Characterization and Cytoprotective Effect of the Hydroethanol Extract from Annona coriacea Mart. (Araticum)

1, 2★, 1, 1, 1, 3, 2, 4, 3, 1, 1, 3, 1, 5, 1, 1, 6, 6, 4, 1★ Corresponding author

  1. 1Department of Biotechnology, Ceará State University, Fortaleza (CE)
  2. 2Department of Biological Chemistry, Regional University of Cariri, Crato (CE)
  3. 3Department of Biomedicine, Leão Sampaio College, Juazeiro do Norte (CE)
  4. 4Department of Medical Microbiology, Federal University of Ceará, Fortaleza (CE)
  5. 5Department of Medicine, Medical School of Juazeiro do Norte - Estacio, Juazeiro do Norte (CE)
  6. 6Department of Health, University of Fortaleza – UNIFOR, Fortaleza (CE) Brazil

CORRESPONDENCE

Henrique D. M. Coutinho

Department of Biological Chemistry, Regional University of Cariri, Crato (CE)

hdmcoutinho@gmail.com

Volume 8, Issue 4 · pp. 253-257 · 2016 · DOI: 10.4103/0974-8490.188882

ABSTRACT

Annona coriacea Mart. (araticum) is a widely distributed tree in the cerrado. Its value is attributed principally to the consumption of its fruit which possesses a large nutritive potential. The objective was to identify the chemical profile and evaluate the antimicrobial and cytoprotective activity of the hydroethanol extract of A. coriacea Mart. (HEAC) leaves against the toxicity of mercury chloride. The characterization of components was carried out using high-performance liquid chromatography (HPLC). The minimum inhibitory concentration (MIC) was determined by microdilution method in broth with strains of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. For evaluation of the modulatory and cytoprotective activity of aminoglycoside antibiotics (gentamicin and amikacin) and mercury chloride (HgCl2), the substances were associated with the HEAC at subinhibitory concentrations (MIC/8). The HPLC analysis revealed the presence of flavonoids such as Luteolin (1.84%) and Quercetin (1.19%) in elevated concentrations. The HEAC presented an MIC ≥512 mg/mL and significant antagonistic action in aminoglycosides modulation, and it also showed cytoprotective activity to S. aureus (significance P < 0.0001) and E. coli (significance P < 0.05) bacteria against the mercury chloride heavy metal with significance, this action being attributed to the chelating properties of the flavonoids found in the chemical identification. The results acquired in this study show that the HEAC presents cytoprotective activity over the tested strains in vitro and can also present antagonistic effect when associated with aminoglycosides, reinforcing the necessity of taking caution when combining natural and pharmaceutical products.

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    Júnior, J. G. A. S., Coutinho, H. D. M., Boris, T. C. C., Cristo, J. S., Pereira, N. L. F., Figueiredo, F. G., Cunha, F. A. B., Aquino, P. E. A., Nascimento, P. A. C., Mesquita, F. J. C., Moreira, P. H. F., Coutinho, S. T. B., Souza, I. T., Teixeira, G. C., Ferreira, N. M. N., Farina, E. O., Torres, C. M. G., Holanda, V. N., Pereira, V. S., & Guedes, M. I. F. (2016). Chemical Characterization and Cytoprotective Effect of the Hydroethanol Extract from Annona coriacea Mart. (Araticum). Pharmacognosy Research, 8(4), 253-257. https://doi.org/10.4103/0974-8490.188882

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    © 2016 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0