Pharmacognosy Research logo
Submit
Pharmacognosy ResearchACADEMIC PUBLISHING
Article

Lactoferrin from Camelus dromedarius Inhibits Nuclear Transcription Factor-kappa B Activation, Cyclooxygenase-2 Expression and Prostaglandin E2 Production in Stimulated Human Chondrocytes

1, 2, 1★★ Corresponding author

  1. 1Departments of Medical Biochemistry
  2. 2Pharmacology and Therapeutics, College of Medicine, Qassim University, Buraidah, KSA

CORRESPONDENCE

Zafar Rasheed

Departments of Medical Biochemistry

zafarrasheed@qumed.edu.sa

Volume 8, Issue 2 · pp. 135-141 · 2016 · DOI: 10.4103/0974-8490.175612

ABSTRACT

Osteoarthritis (OA) is a progressive joint disorder, which remains the leading cause of chronic disability in aged people. Nuclear factor-kappa B (NF)-kB is a major cellular event in OA and its activation by interleukin-1b (IL-1b) plays a critical role in cartilage breakdown in these patients. In this study, we examined the effect of lactoferrin on NF-kB activation, cyclooxygenase-2 (COX-2) expression and prostaglandin E2 (PGE2) production in stimulated human articular chondrocytes. Human chondrocytes were derived from OA articular cartilage and treated with camel lactoferrin and then stimulated with IL-1b. Gene expression was determined by TaqMan assays and protein expression was studied by Western immunoblotting. NF-kB activity and PGE2 levels were determined by ELISA based assays. NF-kB activity was also determined by treatment of chondrocytes with NF-kB specific inhibitor Bay 11–7082. Lactoferrin inhibited IL-1b-induced activation and nuclear translocation of NF-kB p65 in human OA chondrocytes. Lactoferrin also inhibited mRNA/protein expression of COX-2 and production of PGE2. Moreover, Bay 11–7082 also inhibited IL-1b-induced expression of COX-2 and production of PGE2. The inhibitory effect of lactoferrin on the IL-1b induced expression of COX-2 or production of PGE2 was mediated at least in part via suppression of NF-kB activation. Our data determine camel lactoferrin as a novel inhibitor of IL-1b-induced activation of NF-kB signaling events and production of cartilage-degrading molecule PGE2 via inhibition of COX-2 expressions. These results may have important implications for the development of novel therapeutic strategies for the prevention/treatment of OA and other degenerative/inflammatory diseases.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Rasheed, N., Alghasham, A., & Rasheed, Z. (2016). Lactoferrin from Camelus dromedarius Inhibits Nuclear Transcription Factor-kappa B Activation, Cyclooxygenase-2 Expression and Prostaglandin E2 Production in Stimulated Human Chondrocytes. Pharmacognosy Research, 8(2), 135-141. https://doi.org/10.4103/0974-8490.175612

    Copyright and licence

    © 2016 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0