Pharmacognosy Research logo
Submit
Pharmacognosy ResearchACADEMIC PUBLISHING
Article

Cholesterol esterase inhibitory activity of bioactives from leaves of Mangifera indica L

1★, 2, 2, 2, 2, 2★ Corresponding author

  1. 1Research Scholar, Manipal University, Manipal, 2R & D Centre, Natural Remedies Pvt Ltd, Bengaluru, India
  2. 2R & D Centre, Natural Remedies Pvt Ltd, Bengaluru, India

CORRESPONDENCE

G. M. Gururaja

Research Scholar, Manipal University, Manipal

gururajphyto@gmail.com

Volume 7, Issue 4 · pp. 355-362 · 2015 · DOI: 10.4103/0974-8490.159578

ABSTRACT

In the earlier studies, methanolic extract of Mangifera indica L leaf was exhibited hypocholesterol activity. However, the bioactive compounds responsible for the same are not reported so far. To isolate the bioactive compounds with hypocholesterol activity from the leaf extract using cholesterol esterase inhibition assay which can be used for the standardization of extract. The leaf methanolic extract of M. indica (Sindoora variety) was partitioned with ethyl acetate and chromatographed on silica gel to yield twelve fractions and the activity was monitored by using cholesterol esterase inhibition assay. Active fractions were re-chromatographed to yield individual compounds. A major compound mangiferin present in the extract was screened along with other varieties of mango leaves for cholesterol esterase inhibition assay. However, the result indicates that compounds other than mangiferin may be active in the extract. Invitro pancreatic cholesterol esterase inhibition assay was used for bioactivity guided fractionation (BAGF) to yield bioactive compound for standardization of extract. Bioactivity guided fractionation afford the active fraction containing 3b-taraxerol with an IC50 value of 0.86µg/ml.Conclusion: This study demonstrates that M.indica methanol extract of leaf have significant hypocholesterol activity which is standardized with 3b-taraxerol, a standardized extract for hypocholesterol activity resulted in development of dietary supplement from leaves of Mangifera indica.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Gururaja, G. M., Mundkinajeddu, D., Dethe, S. M., Sangli, G. K., Abhilash, K., & Agarwal, A. (2015). Cholesterol esterase inhibitory activity of bioactives from leaves of Mangifera indica L. Pharmacognosy Research, 7(4), 355-362. https://doi.org/10.4103/0974-8490.159578

    Copyright and licence

    © 2015 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0