Analytical Characterization of Achyranthes aspera L. Whole Plant

ABSTRACT

The therapeutic properties of Achyranthes aspera have long been acknowledged and integrated into diverse traditional healthcare like Ayurvedic, Siddha, Unani and Naturopathic medicine for the management of various ailments. In ancient Indian systems of medicine such as Siddha and Ayurveda, the constituents found in this medicinal plant play a pivotal role in treating disorders. Both macro and micro elements are crucial components for plants to carry out their basic functions. Consequently, the ash of Achyranthes aspera was analyzed chemically, with a focus on phytochemical and chromatographic analysis, along with spectral characterization of the entire plant, to determine the presence of inorganic constituents. Standard methods were followed for all physicochemical parameters. Chromatographic characterization was carried out using High Performance Thin Layer Chromatography (HPTLC) and High-Performance Liquid Chromatography (HPLC). Additionally, elemental characterization was performed using different instruments such as Energy Dispersive X-ray analysis (EDX), X-ray Fluorescence (XRF), Powder X-ray Diffraction (PXRD), X-ray Photoelectron Spectroscopy (XPS) and Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES) to investigate the minerals present in the ash of Achyranthes aspera. The HPTLC photo documentation revealed the presence of five spots at 254 nm and eight spots at 366 nm. Vanillin sulfuric acid reagent derivatization resulted in the appearance of 9 spots under white light. Furthermore, the HPLC chromatogram showed the separation of 13 peaks at 366 nm. The evaluation techniques also detected various elements such as Carbon (C), Oxygen (O), Sodium (Na), Magnesium (Mg), Aluminium (Al), Silicon (Si), Phosphorus (O), sulphur (S), Chlorine (Cl), potassium (K), Calcium (Ca), Manganese (Mn), Chromium (Cr), Iron (Fe) and Zinc (Zn). The analysis primarily focuses on quality control criteria and the mineral composition of the plant.

KEYWORDS

• Achyranthes aspera
• Nayurivi
• SEM-EDX
• XRF
• XPS
• PXRD
• ICP-OES

Showing references and in-text citations exactly as published.

• DOI10.5530/pres.20251928Copy

Copyright and licence

© 2025 Author(s)

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0

RELATED ARTICLES

Matched on section and article type within this issue of PR.

• Article · VOLUME 17, ISSUE 1Quality Standards of Athiyadhi Kashayam - A Polyherbal Classical Siddha Formulation with Potent Anti-Diabetic IngredientsMeenakshi Chandrasekaran, Susikumar Sundharamoorthy, Sunil Kumar Koppala Narayana, Bharath Kumar Govindaraju · pp. 31-41Original ArticleSame type: Article
• Article · VOLUME 17, ISSUE 1Antimicrobial Potential of Flavonoids (Free and Bound) of Euphorbia hirta against Selected Dermatophytes StrainsBindu Sharma, Vijay Laxmi Songer, Vinay Kumar Meena · pp. 42-52Original ArticleSame type: Article
• Article · VOLUME 17, ISSUE 1Profiling of Calcium Content among the Leafy Vegetables of West Bengal Using Atomic Absorption SpectroscopyDurba Banerjee, Palash Mondal, Prerona Saha · pp. 53-59Original ArticleSame type: Article
• Article · VOLUME 17, ISSUE 1Ethanolic Extract of aerial parts of Achyranthes aspera Attenuates Hepatotoxicity and Nephrotoxicity Induced by Cisplatin in RatArijit Chaudhuri, Udichi Kataria, Anurag Dubey · pp. 60-69Original ArticleSame type: Article