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Anthelmintic Activity of Hypericum japonicum Thunb.: An in vitro and in silico Studies

1, 1★★ Corresponding author

  1. 1Department of Zoology, Pharmacology and Bioinformatics Laboratory, Bodoland University, Kokrajhar, Assam, INDIA.

CORRESPONDENCE

Ananta Swargiary

Department of Zoology, Pharmacology and Bioinformatics Laboratory, Bodoland University, Kokrajhar, Assam, INDIA.

ananbuzoo101@gmail.com

Received: 18-11-2024; Accepted: 09-01-2025.

Volume 17, Issue 1 · pp. 283-294 · 2025 · DOI: 10.5530/pres.20252026

ABSTRACT

Hypericum japonicum Thunb. is a vital medicinal plant in Northeast India, traditionally used by tribal communities to treat helminth infections with leaf extracts. The present study investigates the in vitro and in silico studies of anthelmintic activity of H. japonicum. The anthelmintic activity was tested on Paramphistomum sp. with a test dose of 5 mg/mL to see the paralysis and death times. Phytocompounds were identified using GC-MS technique. 5 key enzymes-Alkaline Phosphatase (ALP), Acid Phosphatase (ACP), malate-and lactate dehydrogenase, and acetylcholinesterases were assayed using standard protocol. Furthermore, identified compounds were studied for their binding activity with the enzymes. Diethyl ether extract of H. japonicum showed the most potent anthelmintic activity against Paramphistomum sp. in the present study. GC-MS analysis identified 12 compounds in the diethyl ether extract. Of the five enzymes studied, ALP showed highest reduction (42.59%) and the least was found in ACP (16.21%) compared to control. Molecular docking observed strongest binding affinity between compound-2 and AchE (-6.73 kcal/mol) followed by ALP, ACP, and MDH enzymes. The findings suggest that Hypericum japonicum could be a potential source of anthelmintic agents, warranting further studies to elucidate its exact mechanism of action.

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    Roy, M. K., & Swargiary, A. (2025). Anthelmintic Activity of Hypericum japonicum Thunb.: An in vitro and in silico Studies. Pharmacognosy Research, 17(1), 283-294. https://doi.org/10.5530/pres.20252026

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    © 2025 Author(s)

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