Pharmacognosy Research logo
Submit
Pharmacognosy ResearchACADEMIC PUBLISHING
Article

Exploring the Glycosidic Compounds in Ipomoea sagittifolia Stems: A High-Performance Liquid Chromatography Approach

1★, 2, 3, 4, 3, 1, 1, 1★ Corresponding author

  1. 1Department of Pharmacognosy, V. V. Institute of Pharmaceutical Sciences, Seshadri Rao Knowledge Village, Gudlavalleru, Krishna District, Andhra Pradesh, INDIA.
  2. 2Faculty of Pharmaceutical Sciences, Narayana Pharmacy College, Chinthareddypalem, Nellore, SPSR District, Andhra Pradesh, INDIA.
  3. 3Department of Pharmacy, K. M. College of Pharmacy, The Tamilnadu Dr. M. G. R. Medical University, Madurai, Tamil Nadu, INDIA.
  4. 4Department of Pharmacognosy, Arulmigu Kalasalingam College of Pharmacy, Anand Nagar, Krishnan Kovil, Tamil Nadu, INDIA.

CORRESPONDENCE

Raveesha Peeriga

Department of Pharmacognosy, V. V. Institute of Pharmaceutical Sciences, Seshadri Rao Knowledge Village, Gudlavalleru, Krishna District, Andhra Pradesh, INDIA.

drprsha@gmail.com

Received: 04-10-2024; Accepted: 03-12-2024.

Volume 17, Issue 1 · pp. 162-172 · 2025 · DOI: 10.5530/pres.20251964

ABSTRACT

The abstract provides a comprehensive overview of the study on secondary metabolites from Ipomoea sagittifolia, emphasizing the extraction, isolation and characterization processes. Secondary metabolites, including alkaloids, flavonoids, phenols and glycosides, were identified and analyzed using various analytical techniques. Ethanol extraction followed by Soxhlet apparatus yielded a crude extract, subsequently purified through column chromatography using a solvent mixture of n-butanol, glacial acetic acid and water. Thin Layer Chromatography (TLC) confirmed the presence of compounds across 108 eluted fractions, further validated by UV-vis spectroscopy revealing absorption peaks characteristic of glycosides at 288 nm. Fourier Transform Infrared (FTIR) spectroscopy highlighted functional groups such as alcohols, phenols, carboxylic acids and ketones, typical of glycoside structures. High-Performance Liquid Chromatography (HPLC) confirmed the presence of cardiac glycosides through retention time analysis, aligning closely with Scillaren A standards. These findings underscore the pharmacological potential of Ipomoea sagittifolia in traditional medicine and pharmaceutical applications, supported by its diverse secondary metabolite profile. Future research could delve deeper into bioactivity assays to elucidate specific therapeutic properties linked to these metabolites, thereby enhancing its relevance in healthcare and biotechnological innovations.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Peeriga, R., Manubolu, K., Somasundaram, J., Arabath, S. A. M. S., Petchimuthu, S., Manepalli, S. N. J., Maddela, V. K., & Makireddy, K. (2025). Exploring the Glycosidic Compounds in Ipomoea sagittifolia Stems: A High-Performance Liquid Chromatography Approach. Pharmacognosy Research, 17(1), 162-172. https://doi.org/10.5530/pres.20251964

    Copyright and licence

    © 2025 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0