Modulating Effect of Hypnea musciformis (Red Seaweed) on Lipid Peroxidation, Antioxidants and Biotransforming Enzymes in 7,12‑Dimethylbenz (a) Anthracene Induced Mammary Carcinogenesis in Experimental Animals
1, 1, 2, 2
- 1Department of Botany, Division of Algal Biotechnology, Annamalai University, Annamalainagar, Tamil Nadu, INDIA.
- 2Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, Tamil Nadu, INDIA.
CORRESPONDENCE
Kathiresan Sivakumar
Department of Botany, Division of Algal Biotechnology, Annamalai University, Annamalainagar, Tamil Nadu, INDIA.
Volume 9, Issue 1 · pp. 108-115 · 2017 · DOI: 10.4103/0974-8490.187085
ABSTRACT
Background: Breast cancer is the second most widespread diagnosed cancer and second leading cause of cancer death in women.Objective: The present work was carried out to evaluate the chemo preventive potential of Hypnea musciformis (ethanol extract) seaweed on oxidative stress markers, bio transforming enzymes, incidence of tumors, and pathological observation in 7,12-dimethylbenzanthracene (DMBA) exposed experimental mammary carcinogenesis.Materials and Methods: Female Sprague–Dawley rats were randomly divided into four groups. Rats in the group 1 served as control. Rats in the group 2 and 3 received a single subcutaneous injection of DMBA (25 mg/kg body weight (b.w)) in the mammary gland to develop mammary carcinoma. In addition, group 3 rats were orally administrated with 200 mg/kg between of H. musciformis along with DMBA injection and group 4 rats received ethanolic extract of H. musciformis every day orally (200 mg/kg b.w) throughout the experimental period of 16 weeks.Results: Our results revealed that treatment with H. musciformis ethanolic extract to DMBA treated rats significantly reduced the incidence of tumor and tumor volume as compared to DMBA alone treated rats. Moreover, our results showed imbalance in the activities/levels of lipid peroxidation by products, antioxidant enzymes, and bio transforming phase I and II enzymes in the circulation, liver and mammary tissues of DMBA treated rats which were significantly modulated to near normal on treatment with ethanolic extract of H. musciformis. All these alterations were supported by histochemical findings.Conclusion: The results obtained from this study suggest that chemo preventive potential of H. musciformis ethanol extract is probably due to their free radicals quenching effect and modulating potential of bio transforming enzymes during DMBA exposed experimental mammary carcinogenesis.
KEYWORDS
REFERENCES
As publishedShowing references and in-text citations exactly as published.
Cite this article
SELECT FORMAT
Balamurugan, M., Sivakumar, K., Mariadoss, A. V. A., & Suresh, K. (2017). Modulating Effect of Hypnea musciformis (Red Seaweed) on Lipid Peroxidation, Antioxidants and Biotransforming Enzymes in 7,12‑Dimethylbenz (a) Anthracene Induced Mammary Carcinogenesis in Experimental Animals. Pharmacognosy Research, 9(1), 108-115. https://doi.org/10.4103/0974-8490.187085
Copyright and licence
© 2017 Author(s)
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0