Pharmacognosy Research logo
Submit
Pharmacognosy ResearchACADEMIC PUBLISHING
Article

Anti-inflammatory Activity of Berry Fruits in Mice Model of Inflammation is Based on Oxidative Stress Modulation

1, 1, 2, 2, 2, 2, 1, 3, 3, 4, 3, 2★★ Corresponding author

  1. 1Laboratory of Pharmacology, Life Sciences Branch, University of the West of Santa Catarina – Unoesc
  2. 2Post Graduate Program in Science and Biotechnology, Branch of Biotechnology, University of the West of Santa Catarina – Unoesc
  3. 3Post Graduate Program in Pharmaceutical Sciences and Chemical-Pharmaceutical Investigations Center, University of Itajaí Valley - Univali
  4. 4Laboratory of Infectious and Parasitic Diseases, Life Sciences Branch, University of the West of Santa Catarina – Unoesc, Brazil

CORRESPONDENCE

Claudriana Locatelli

Post Graduate Program in Science and Biotechnology, Branch of Biotechnology, University of the West of Santa Catarina – Unoesc

claudrilocatelli@gmail.com

Volume 8, Issue 5s · pp. S42-S49 · 2016 · DOI: 10.4103/0974-8490.178642

ABSTRACT

Many fruits have been used as nutraceuticals because the presence of bioactive molecules that play biological activities. The present study was designed to compare the anti-inflammatory and antioxidant effects of methanolic extracts of Lycium barbarum (GOJI), Vaccinium macrocarpon (CRAN) and Vaccinium myrtillus (BLUE). Mices were treated with extracts (50 and 200 mg/kg, p.o.), twice a day through 10 days. Phytochemical analysis was performed by high-performance liquid chromatography. Antioxidant activity was determine by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, reducing power, lipid peroxidation thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH) and catalase (CAT) activity. Anti-inflammatory activity was evaluated by paw edema followed by determination of myeloperoxidase (MPO) and TBARS. High amount of phenolic compounds, including rutin, were identified in all berries extracts. However, quercetin was observed only in BLUE and CRAN. GOJI presents higher scavenging activity of DPPH radical and reducing power than BLUE and CRAN. The extracts improved antioxidant status in liver; BLUE showed the largest reduction (75.3%) in TBARS when compared to CRAN (70.7%) and GOJI (65.3%). Nonetheless, CAT activity was lower in BLUE group. However, hepatic concentrations of GSH were higher in animals treated with GOJI rather than CRAN and BLUE. Despite all fruits caused a remarkable reduction in paw edema and TBARS, only BLUE and CRAN were able to reduce MPO. These results suggest that quercetin, rutin, or other phenolic compound found in these berry fruits extracts could produce an anti-inflammatory response based on modulation of oxidative stress in paw edema model.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Nardi, G. M., Januario, A. G. F., Freire, C. G., Megiolaro, F., Schneider, K., Perazzoli, M. R. A., Nascimento, S. R. D., Gon, A. C., Mariano, L. N. B., Wagner, G., Niero, R., & Locatelli, C. (2016). Anti-inflammatory Activity of Berry Fruits in Mice Model of Inflammation is Based on Oxidative Stress Modulation. Pharmacognosy Research, 8(5s), S42-S49. https://doi.org/10.4103/0974-8490.178642

    Copyright and licence

    © 2016 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0