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Evaluation of In Vitro Cytotoxic and Antioxidant Activity of Datura metel Linn. and Cynodon dactylon Linn. Extracts

1★, 2, 1★ Corresponding author

  1. 1Department of Virology, Ha~kine Institute for Training, Research and Testing, Parel
  2. 2Department of Biochemistry, The Institute of Science, Fort, Mumbai, Maharashtra, India

CORRESPONDENCE

Soumen Roy

Department of Virology, Ha~kine Institute for Training, Research and Testing, Parel

soumenroy118@gmail.com

Volume 8, Issue 2 · pp. 123-127 · 2016 · DOI: 10.4103/0974-8490.175610

ABSTRACT

To evaluate in vitro cytotoxicity and antioxidant activity of Datura metel L. and Cynodon dactylon L. extracts. The extraction of plants parts (datura seed and fruit pulp) and areal parts of durva was carried out using soxhlet and cold extraction method using solvents namely methanol and distilled water. The total phenolic content (TPC) and total flavonoid content (TFC) was determined by established methods. The in vitro cytotoxicity assay was performed in vero cell line by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay method. In vitro antioxidant activity of the extract was performed by 2, 2-diphenyl-1-picrylhydrazyl radical scavenging method. We found that the highest amount of TPC and TFC in methanolic extracts of seed (268.6 mg of gallic acid equivalence/mg of dry plant material) and fruit pulp (8.84 mg of quercetin equivalence/mg dry plant material) of D. metel, respectively prepared by Soxhlet method. The methanolic extract of C. dactylon prepared using soxhlation has shown potent free radical scavenging activity with 50% inhibitory concentration (IC50) value of 100 mg/ ml. The IC50 of a methanolic cold extract of datura fruit was found to be 3 mg/ml against vero cell line. We observed that plant parts of C. dactylon and D. metel have a high antioxidant activity. Further research is needed to explore the therapeutic potential of these plant extracts.

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    Roy, S., Pawar, S., & Chowdhary, A. (2016). Evaluation of In Vitro Cytotoxic and Antioxidant Activity of Datura metel Linn. and Cynodon dactylon Linn. Extracts. Pharmacognosy Research, 8(2), 123-127. https://doi.org/10.4103/0974-8490.175610

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    © 2016 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0