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Chemical constituents and bioactivities of Glinus oppositifolius

1★, 2, 3, 3, 3, 4, 5★ Corresponding author

  1. 1Department of Chemistry, De La Salle University Science and Technology Complex Leandro V. Locsin Campus, Biñan City, Laguna 4024, 2Departments of Chemistry
  2. 2Biology, De La Salle University, 2401 Taft Avenue, Manila 1004
  3. 3Departments of Chemistry
  4. 4Department of Biology, School of Science and Engineering, Ateneo de Manila University, Loyola Heights, Quezon City 1108, Philippines
  5. 5Division of Chinese Medicinal Chemistry, National Research Institute of Chinese Medicine, Ministry of Health and Welfare, 155-1, Li-Nong St., Sec. 2, Taipei 112, Taiwan

CORRESPONDENCE

Consolacion Y. Ragasa

Department of Chemistry, De La Salle University Science and Technology Complex Leandro V. Locsin Campus, Biñan City, Laguna 4024

consolacion.ragasa@dlsu.edu.ph

Volume 7, Issue 2 · pp. 138-147 · 2015 · DOI: 10.4103/0974-8490.150520

ABSTRACT

To isolate the secondary metabolites from the dichloromethane (DCM) extracts of Glinus oppositifolius; to test for the cytotoxicity of a new triterpene, oppositifolone (1); and to test for the hypoglycemic, analgesic, and antimicrobial potentials of 1, DCM and aqueous leaf extracts of G. oppositifolius. The compounds were isolated by silica gel chromatography and identified by nuclear magnetic resonance spectroscopy. The cytotoxicity potential of 1 was tested using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Triterpene 1, DCM, and aqueous leaf extracts were tested for hypoglycemic potential using the oral glucose tolerance test; analgesic potential using the tail-flick assay, and antimicrobial potential using the disc diffusion method. The DCM extracts of G. oppositifolius afforded 1, squalene, spinasterol, oleanolic acid, phytol, and lutein from the leaves; squalene and spergulagenin A from the stems; and spinasterol from the roots. Triterpene 1 was cytotoxic against human colon carcinoma 116 with an IC50 value of 28.7 but did not exhibit cytotoxicity against A549. The aqueous leaf extract at 200 mg/kg body weight (BW) exhibited hypoglycemic activity with a pronounced % blood glucose reduction of 70.76% ±17.4% within 0.5 h after introduction. The DCM leaf extract showed a lower % blood glucose reduction of 18.52% ±13.5% at 200 mg/kg BW within 1.5 h after introduction, while 1 did not exhibit hypoglycemic activity. The samples did not exhibit analgesic property and were inactive against multiple drug resistant bacterial pathogens. The compounds responsible for the hypoglycemic activity of G. oppositifolius which are fast acting (0.5 h) are found in the aqueous leaf extract.

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    Ragasa, C. Y., Cabrera, E. C., Torres, O. B., Buluran, A. I., Espineli, D. L., Raga, D. D., & Shen, C. (2015). Chemical constituents and bioactivities of Glinus oppositifolius. Pharmacognosy Research, 7(2), 138-147. https://doi.org/10.4103/0974-8490.150520

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    © 2015 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0