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Isolation and identification of bacterial endophytes from pharmaceutical agarwood-producing Aquilaria species

1★, 1, 2★ Corresponding author

  1. 1Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Bedong Semeling Road, Semeling 08100, Kedah
  2. 2Tissue Culture Laboratory, Forest Research Institute Malaysia 52109 Kepong, Selangor, 2Malaysian Biotechnology Corporation, Level 23, Menara Atlan 161B, Jalan Ampany 50450 Kuala Lumpur, Malaysia

CORRESPONDENCE

Subhash J. Bhore

Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Bedong Semeling Road, Semeling 08100, Kedah

subhash@aimst.edu.my

Volume 5, Issue 2 · pp. 134-138 · 2013 · DOI: 10.4103/0974-8490.110545

ABSTRACT

Resins and gums are used in traditional medicine and do have potential applications in pharmacy and medicine. Agarwood is the fragrant resinous wood, which is an important commodity from Aquilaria species and has been used as a sedative, analgesic, and digestive in traditional medicine. Endophytic bacteria are potentially important in producing pharmaceutical compounds found in the plants. Hence, it was important to understand which types of endophytic bacteria are associated with pharmaceutical agarwood-producing Aquilaria species. This study was undertaken to isolate and identify endophytic bacteria associated with agarwood-producing seven (7) Aquilaria species from Malaysia. Botanical samples of seven Aquilaria species were collected, and endophytic bacteria were isolated from surface-sterilized-tissue samples. The 16S rRNA gene fragments were amplified using PCR method, and endophytic bacterial isolates (EBIs) were identified based on 16S rRNA gene sequence similarity based method. Culturable, 77 EBIs were analyzed, and results of 16S rRNA gene sequences analysis suggest that 18 different types of endophytic bacteria are associated with (seven) Aquilaria species. From 77 EBIs, majority (36.4%) of the isolates were of Bacillus pumilus. These findings indicate that agarwood-producing Aquilaria species are harboring 18 different types of culturable endophytic bacteria.

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    Bhore, S. J., Preveena, J., & Kandasamy, K. I. (2013). Isolation and identification of bacterial endophytes from pharmaceutical agarwood-producing Aquilaria species. Pharmacognosy Research, 5(2), 134-138. https://doi.org/10.4103/0974-8490.110545

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    © 2013 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0