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A validated UV-HPLC method for determination of chlorogenic acid in Lepidogrammitis drymoglossoides (Baker) Ching, Polypodiaceae

1, 2, 3, 2, 2, 1★★ Corresponding author

  1. 1School of Biological Science and Technology, Central South University, Changsha
  2. 2College of Pharmacy, Central South University, Changsha
  3. 3National Hepatobiliary and Enteric Surgery Research Center of The Ministry of Health, Xiangya Hospital, Changsha, People’s Republic China

CORRESPONDENCE

Yuxiang Chen

School of Biological Science and Technology, Central South University, Changsha

chenyx@xysm.net

Volume 4, Issue 3 · pp. 148-153 · 2012 · DOI: 10.4103/0974-8490.99076

ABSTRACT

Lepidogrammitis drymoglossoides (Baker) Ching (L. drymoglossoides), a member of the Polypodiaceae family, was used in the treatment of numerous diseases. However, none of the potential ingredients and the quality control methods concerning this plant medicine was pronounced. To identify chlorogenic acid (CGA) from L. drymoglossoides and develop a high performance liquid chromatography (HPLC) assay of CGA. UV, TLC, and HPLC were utilized to identify the phytochemicals of L. drymoglossoides and determine the CGA content, respectively. The HPLC conditions were as following: a Phenomenex Luna C18 (2) (250 × 4.6 mm i.d.; 5 μm particle size; 100 Å pore size) column; the mobile phase of the mixture of acetonitrile and 0.5% aqueous phosphoric acid (11.5:88.5 v/v); the flow rate of 1.0 mL/min and determination wavelength of 327 nm. The proposed HPLC method has been developed and validated. The calibration curve was y = 28328x + 16610 (R2 = 0.9997). The intra-day and inter-day precision and intermediate precision were validated with the RSD less than 5%. The mean recovery rate of the method ranged from 95% to 104%, with the RSD less than 5%. The LOD and LQD values were 0.049 and 0.132 mg/L, respectively. The content of CGA in L. drymoglossoides approximately reached 0.24% (v/v) by the proposed extraction and determination methods. The assay method was simple, convenient, and accurate to the quantification of CGA and can be used for the quality control of the herb.

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    Wen, J., Kang, L., Liu, H., Xiao, Y., Zhang, X., & Chen, Y. (2012). A validated UV-HPLC method for determination of chlorogenic acid in Lepidogrammitis drymoglossoides (Baker) Ching, Polypodiaceae. Pharmacognosy Research, 4(3), 148-153. https://doi.org/10.4103/0974-8490.99076

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    © 2012 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0