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Chitin Derivative from Marine Source Artemia franciscana Cysts Inhibits Colorectal Adenocarcinoma Cells by Regulating Apoptotic Mediators

1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 2

  1. 1Quaid-e-Millath Government College for Women, University of Madras, Tamil Nadu, INDIA.
  2. 2Institute of Marine Biology, National Taiwan Ocean University, Keelung, TAIWAN.
  3. 3Department of Mechanical Engineering, Indian Institute of Technology Madras, Chennai, Tamil Nadu, INDIA.
  4. 4Department of Chemical Engineering, Algappa college of technology, Anna University, Chennai, Tamil Nadu, INDIA.
  5. 5Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai, Tamil Nadu, INDIA.
  6. 6Department of Biochemistry, Saveetha Medical College and Hospital, Saveetha Institute of Medical and Technical Sciences (Deemed to be University), Thandalam, Chennai, Tamil Nadu, INDIA., 8School of Sciences, Department of Microbiology, P P. Savani University, Dhamdod, Kosamba, Surat, Gujarat, INDIA.
  7. 7Department of Biochemistry, Saveetha Medical College and Hospital, Saveetha Institute of Medical and Technical Sciences (Deemed to be University), Thandalam, Chennai, Tamil Nadu, INDIA.
  8. 8School of Sciences, Department of Microbiology, P P. Savani University, Dhamdod, Kosamba, Surat, Gujarat, INDIA.
  9. 9Department of Biochemistry, PSG College of Arts and Science, Coimbatore, Tamil Nadu, INDIA.
  10. 10Department of Biotechnology, PSG College of Arts and Science, Coimbatore, Tamil Nadu, INDIA.

CORRESPONDENCE

Jiang-Shiou Hwang

[1]Institute of Marine Biology, National Taiwan Ocean University, Keelung-202301, TAIWAN. and [2]Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung-202301, TAIWAN.

jshwang@mail.ntou.edu.tw

Received: 18-02-2025; Accepted: 06-06-2025.

Volume 17, Issue 3 · pp. 973-982 · 2025 · DOI: 10.5530/pres.20252138

ABSTRACT

Chitin is widely preferred in biomedical application due to its biocompatible and biodegradable nature. Naturally occurring chitin is a potential antimicrobial agent and plays a major role in degrading outer shell wall of the lower crustaceans. Naturally occurring sources of chitin are more suitable for oncological research. In the present study, the chitin derivative isolated from marine source (Artemia franciscana cysts) is evaluated for its potential to act as a therapeutic target for colorectal adenocarcinoma. The FTIR and X-ray diffraction analysis of the isolated chitin has confirmed the presence of characteristic functional groups such as amide groups, hydroxyl groups and glycosidic linkages along with their material's crystalline structure. The anticancer activity of extracted chitin derivative showed significant cytotoxic effect in this study which could be attributed to their positively charged functional groups. Further mRNA gene expression analysis has revealed that the chitin derivative also exhibited apoptotic potential against HT29 cell lines by upregulating caspase-3 and caspase-9 gene and by down regulation of B cell lymphoma-2 (Bcl-2) gene expressions. In conclusion the present study showed that the Artemia sp. cyst-mediated chitin derivative could be a potential anticancer agent against colorectal adenocarcinoma through their anti-proliferative activity and by activation of apoptotic pathway in HT29 cell lines.

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    Poovazhagi, R., Thirunavukkarasu, S., Santhosh, J. S., Lakshmanan, K., Javee, A., Dhanashekaran, C. J. M., Radhakrishnan, N., Prabhu, V. V., Sakthivel, K. M., Rasmi, R. R., & Hwang, J. (2025). Chitin Derivative from Marine Source Artemia franciscana Cysts Inhibits Colorectal Adenocarcinoma Cells by Regulating Apoptotic Mediators. Pharmacognosy Research, 17(3), 973-982. https://doi.org/10.5530/pres.20252138

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    © 2025 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0