GC-MS Analysis of Bio-Active Compounds in Hydroethanolic Extract of Caesalpinia sappan

Abstract

The presence of phytochemical constituents has been reported from species of the Compositae Caesalpiniaceae. The present study was designed to determine the bioactive compounds in the heartwood of hydroethanolic extract. Phytochemical screening of the entire herb of Caesalpinia sappan revealed the presence of some bioactive components. Gas Chromatography-Mass Spectrometry (GC-MS) analysis of the heartwood of hydroethanolic extract was performed on GC-MS equipment. The phytochemical tests showed the presence of alkaloids, alkaloids, cardiac glycosides, flavonoids, phenols, phlorotannins, reducing sugars, saponins, steroids, tannins, terpenoids, volatile oils, carbohydrates, protein/amino acids in the hydroethanolic extract of Caesalpinia sappan. The GC-MS analysis has shown the presence of different phytochemical compounds in the hydroethanolic extract of Caesalpinia sappan. A total of 38 compounds were identified. The major components are 9-octadecenoic acid, Oleic Acid, Sarcosine and Dinonanoin. From the results, it is evident that Caesalpinia sappan contains various photo components and is recommended as a plant of phytopharmaceutical importance.

Keywords: Caesalpinia sappan (CS); Heartwood hydroethanolic extraction; Phytochemistry; GC-MS analysis