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Screening and Evaluation of Potential Antifungal Plant Extracts against Skin Infecting Fungus Trichophyton rubrum

1, 2

  1. 1Department of Biotechnology, St. Jospeh’s College of Arts and Science for Women, Hosur, Tamil Nadu, INDIA.
  2. 2Department of Biotechnology, Arignar Anna Arts and Science College, Krishnagiri, Tamil Nadu, INDIA.

CORRESPONDENCE

Caroline Rose J

Department of Biotechnology, Arignar Anna Arts and Science College, Krishnagiri, Tamil Nadu, INDIA.

jcarolinerose@gmail.com

Received: 12-12-2022; Accepted: 11-01-2023.

Volume 15, Issue 2 · pp. 328-337 · 2023 · DOI: 10.5530/pres.15.2.035

ABSTRACT

Dermatophytosis is mainly caused by Trichophyton rubrum. The use of presently available conventional antifungal agents is limited due to various side-effects, requirement of long-term application, emergence of resistant organisms and cost-effectiveness. In the present study, methanolic extract of 12 medicinal plants was screened for antifungal activity against T. rubrum. To identify the phytochemicals in the extract qualitative and quantitative analysis was performed along with minimum inhibitory concentration (MIC). By analysing the samples using gas chromatography-mass spectrometry (GC-MS), the chemicals that were present were identified. Among the various plants studied, the methanolic extracts of Aegle marmelos leaves (AML-ME) and Pimpinella anisum seed (PMS-ME) showed high zone of inhibition of 16 and 22 mm, respectively. MIC of both the extracts was found to be 100 μg/ml. Some major compounds identified in AML-ME with alkaloids are N, N,-dimethyl-o-(1-methyl-butyl)-hydroxylamine, 13-tetradece-11-yn-1-ol (15.21%), n-hexadecanoic acid, Propanal, 2,3-dihydroxy-, (s)-(5.22%) and 1,3-butanediol, and in PAS-ME, hexamethylene chloroiodide, cyclopentene,3-hexyl, E-2-octadecadecen-1-ol, n-hexadecanoic acid and 2-cyclopentene-1-undecanoic acid, ethyl ester. Both AML-ME and PAS-ME showed dose-dependent in vitro antioxidant activity against the radicals such as DPPH and ABTS. Also, FRAP activity was found in both the extracts. Altogether, the presence of antifungal and antioxidant activity in the AML-ME and PAS-ME might be advantageous in exploring these extracts as either alternative or complementary therapy to the available antifungal agents further. The phytochemicals present in these extracts has acted synergistically against the T. rubrum and affected its viability.

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    A, M. K., & J, C. R. (2023). Screening and Evaluation of Potential Antifungal Plant Extracts against Skin Infecting Fungus Trichophyton rubrum. Pharmacognosy Research, 15(2), 328-337. https://doi.org/10.5530/pres.15.2.035

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    © 2023 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0