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Cholinergic‑Enhancing and Antioxidant Effect of Vigna subterranea (L.) Verdc. (Fabaceae) Landrace Aqueous Extract on Scopolamine‑Induced Amnesia in Male Swiss Mice

1, 1, 1, 1, 1, 1, 2, 3

  1. 1Department of Biological Science, Faculty of Science, Laboratory of Cognitive and Behavioral Neuroscience, University of Maroua, Maroua, ROMANIA.
  2. 2Center of Biomedical Research of The Romanian Academy, Iasi Branch, Alexandru Ioan Cuza University, Iasi, ROMANIA.
  3. 3Department of Biological Science, Faculty of Science, Laboratory of Cognitive and Behavioral Neuroscience, University of Maroua, Maroua; Department of Biological Science, Faculty of Science, Laboratory of Medicinal Plants, Health and Galenic Formulations, University of Ngaoundéré, Ngaoundéré, CAMEROON.

CORRESPONDENCE

Harquin Simplice Foyet

Department of Biological Science, Faculty of Science, Laboratory of Cognitive and Behavioral Neuroscience, University of Maroua, Maroua, ROMANIA.

fharquins@gmail.com

Received: 16-06-2019; Accepted: 09-01-2020.

Volume 12, Issue 3 · pp. 303-312 · 2020 · DOI: 10.4103/pr.pr_62_19

ABSTRACT

Background: Currently, no drug effectively curbs down the progression of Alzheimer’s disease (AD), and thus early prevention is important.Objectives: The present study was conducted to investigate the protective effects of the aqueous extract of Vigna subterranea seed landrace on amnesia induced by scopolamine in mice.Materials and Methods: V. subterranea aqueous extract (100, 200, and 400 mg/kg BW) was administered by gavage for 9 consecutive days and memory impairment was induced by repeated intraperitoneal injection of scopolamine (1.5 mg/kg). The Y‑maze (YM), Morris water maze (MWM), novel object recognition paradigm (NOR), and the T maze™ were used to assess learning, memory, and retention. Superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA) levels, and acetyclcholine esterase activity were also evaluated in the mouse hippocampi homogenates.Results: V. subterranea aqueous extract (400 mg/kg) significantly increased the percentage of spontaneous alternation in the YM task and decreased escape latency in the MWM. Moreover, this dose brought about a significant improvement in the time spent in the preferred TM arm and discrimination index in the NOR tasks despite repeated scopolamine injection. Additionally, low acetylcholine esterase levels, reduced lipid peroxidation (MDA), but increased antioxidant enzyme (CAT and SOD) activity were observed in hippocampi homogenate of mice pretreated with the extract. A protective action against hippocampal cell damage was also evident.Conclusion: This finding suggests that the aqueous extract of V. subterranea seed landrace may improve learning and memory.

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    Abaissou, H. H. N., Foyet, H. S., Wado, E. K., Nkwingwa, B. K., Kamda, J. L. D., Djomessie, L. B. K., Ciobica, A., & Bum, E. N. (2020). Cholinergic‑Enhancing and Antioxidant Effect of Vigna subterranea (L.) Verdc. (Fabaceae) Landrace Aqueous Extract on Scopolamine‑Induced Amnesia in Male Swiss Mice. Pharmacognosy Research, 12(3), 303-312. https://doi.org/10.4103/pr.pr_62_19

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    © 2020 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0