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Antioxidant and Cytotoxic Potential of Endophytic Fungi Isolated from Medicinal Plant Tragia involucrata L.

1, 1, 1, 2, 3

  1. 1Department of Studies and Research in Biochemistry, PG Centre, Mangalore University, Kodagu, INDIA.
  2. 2Department of Biochemistry, Davanagere University, Davanagere, INDIA.
  3. 3Department of Biotechnology, A Constituent College of University of Agriculture, Sciences, Bangalore, INDIA.

CORRESPONDENCE

Chandrashekhar G. Joshi

Department of Studies and Research in Biochemistry, PG Centre, Mangalore University, Kodagu, INDIA.

josheejoshee@gmail.com

Volume 10, Issue 2 · pp. 188-194 · 2018 · DOI: 10.4103/pr.pr_137_17

ABSTRACT

Objective: The aim of the present study was to test the antioxidant and cytotoxic and DNA protection activities of ethyl acetate extracts of endophytic fungi isolated from Tragia involucrata Linn. (Euphorbiaceae).Materials and Methods: The 1, 1‑diphenyl‑2‑picrylhydrazyl scavenging, reducing power, and total antioxidant assay were used to evaluate the antioxidant activity. Cytotoxic activity of endophytic fungal extracts against MCF‑7 and MOLT‑4 cell lines was carried out using 3‑(4, 5‑dimethylthiazol‑2‑yl)‑2, 5‑diphenyl‑2H‑tetrazolium bromide method.Results: We have successfully isolated four endophytic fungi from T. involucrata, namely Penicillium citrinum CGJ‑C1 (GenBank accession No. KT780618), P. citrinum CGJ‑C2 (GenBank accession No. KP739821), Cladosporium sp. CGJ‑D1 (GenBank accession No. KP739822), and Cryptendoxyla hypophloia CGJ‑D2 (GenBank accession No. KT780619). The ethyl acetate extract of P. citrinum CGJ‑C2 showed the highest antioxidant as well as cytotoxic activity among the four fungal extracts taken for the study. All the extracts showed moderate DNA protection activity.Conclusions: Further studies on the isolation and purification of the lead molecule will help in designing the novel therapy for different ailments associated with free radical generation.

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    Danagoudar, A., Joshi, C. G., Ravi, S. K., Kumar, H. G. R., & Ramesh, B. N. (2018). Antioxidant and Cytotoxic Potential of Endophytic Fungi Isolated from Medicinal Plant Tragia involucrata L.. Pharmacognosy Research, 10(2), 188-194. https://doi.org/10.4103/pr.pr_137_17

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    © 2018 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0