Pharmacognosy Research logo
Submit
Pharmacognosy ResearchACADEMIC PUBLISHING
Article

High‑Performance Liquid Chromatography‑Fingerprint Analyses, In vitro Cytotoxicity, Antimicrobial and Antioxidant Activities of the Extracts of Two Cestrum Species Growing in Egypt

1, 2, 2, 3, 2, 4

  1. 1Biochemistry and Molecular Biology Department, Theodor Bilharz Research Institute, EGYPT.
  2. 2Medicinal Chemistry Department, Theodor Bilharz Research Institute, Kornaish El-Nile, Warrak El-Hadar, Imbaba (P.O. 30), EGYPT.
  3. 3Chemistry Department, Faculty of Science, Cairo University, Giza, EGYPT.
  4. 4Microbial Chemistry Department, Genetic Engineering and Biotechnology Division, National Research Centre, El-Bohouth Street 33, Dokki-Giza, EGYPT.

CORRESPONDENCE

Sami M. Nasr

Biochemistry and Molecular Biology Department, Theodor Bilharz Research Institute, EGYPT.

samitbri@gmail.com

Volume 10, Issue 2 · pp. 173-180 · 2018 · DOI: 10.4103/pr.pr_145_17

ABSTRACT

Background: Cancer diseases and microbial resistance are serious health disorders associated with oxidative stress and infectious diseases. Their risks can be reducing via using polyphenols-rich plants.Methodology: Different solvent extracts from two Cestrum species (Cestrum nocturnum and Cestrum elegans) were evaluated for their biological and chemical activities. Also, the chemical profiles of the most promising extracts were investigated via high‑performance liquid chromatography (HPLC)‑fingerprint analyses.Results: The tested extracts showed weak to moderate cytotoxicity against Vero cell line with IC50 values ranged from 133.67 μg/ ml to 57.634 μg/ml. The only noncytotoxic extractive fraction was the dichloromethane extract of C. elegans leaves with an IC50 value of 204.732 μg/ml, while the most toxic extract was the ethyl acetate extract of C. elegans flowers with an IC50 value of 19.22 μg/ml. The antimicrobial activity results revealed that the n‑BuOH extract of C. nocturnum was the most active against four tested microbial strains with inhibition zones (10–13 mm). Also, the water and n‑BuOH extracts of C. elegans leave exhibited moderate activities with inhibition zones (7–9 mm), while for C. elegans flowers both of water and methanol extracts showed strong activities (9–14 mm). In the 2,2’‑diphenyl‑1‑picrylhydrazyl assay, the most active fraction was EtOAc with IC50 values of 100.52 μg/ml and 64.40 μg/ml for C. elegans leaves and flowers respectively, while for C. nocturnum the most active fraction was methanol with an IC50 value of 161.16 μg/ml, all relative to 7.60 μg/ml of ascorbic acid. HPLC‑fingerprint analyses revealed that the major identified compounds in the ethyl acetate extract of C. elegans flowers are caffeic acid, coumaric acid, vanillin, and rutin, while for the n‑butanol extract of C. nocturnum leaves are coumaric acid and vanillin.Conclusion: The obtained results revealed that the two species can be used as natural sources of antioxidant compounds with low cytotoxic effect on the mammalian cell line.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Nasr, S. M., Ghareeb, M. A., Mohamed, M. A., Elwan, N. M., Abdel-Aziz, A. E. A., & Abdel-Aziz, M. S. (2018). High‑Performance Liquid Chromatography‑Fingerprint Analyses, In vitro Cytotoxicity, Antimicrobial and Antioxidant Activities of the Extracts of Two Cestrum Species Growing in Egypt. Pharmacognosy Research, 10(2), 173-180. https://doi.org/10.4103/pr.pr_145_17

    Copyright and licence

    © 2018 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0